Characterization and regulation of ammonium transport systems in Citrus plants
Identifieur interne : 000197 ( France/Analysis ); précédent : 000196; suivant : 000198Characterization and regulation of ammonium transport systems in Citrus plants
Auteurs : M. Cerezo [Espagne] ; P. Tillard [France] ; A. Gojon [France] ; E. Primo-Millo [Espagne] ; P. Garcia-Agustin [Espagne]Source :
- Planta [ 0032-0935 ] ; 2001.
Descripteurs français
- Pascal (Inist)
- Caractérisation, Régulation, Transport biologique, Marquage isotopique, Protéine transport, Site haute affinité, Absorption, Site faible affinité, Nutrition, Ammonium, Nitrate, Proton, Déficit, Métabolisme énergétique, Gradient proton, Gradient concentration, Racine, Citrus sinensis Poncirus trifoliata, Azote 15.
- Wicri :
English descriptors
- KwdEn :
- Absorption, Ammonium, Biological Transport (physiology), Biological transport, Carrier protein, Characterization, Citrus (physiology), Concentration gradient, Deficiency, Down-Regulation, Energy metabolism, High affinity site, Hydrogen-Ion Concentration, Hydroponics, Ionophores, Isotope labelling, Kinetics, Low affinity site, Nitrates, Nitrogen Isotopes, Nutrition, Plant Roots (physiology), Proton, Proton gradient, Proton-Translocating ATPases (antagonists & inhibitors), Quaternary Ammonium Compounds (administration & dosage), Quaternary Ammonium Compounds (antagonists & inhibitors), Quaternary Ammonium Compounds (metabolism), Regulation(control), Root, Up-Regulation.
- MESH :
- chemical , administration & dosage : Quaternary Ammonium Compounds.
- chemical , antagonists & inhibitors : Proton-Translocating ATPases, Quaternary Ammonium Compounds.
- chemical , metabolism : Quaternary Ammonium Compounds.
- chemical : Ionophores, Nitrogen Isotopes.
- physiology : Biological Transport, Citrus, Plant Roots.
- Down-Regulation, Hydrogen-Ion Concentration, Hydroponics, Kinetics, Up-Regulation.
Abstract
We have investigated both the kinetics and regulation of 15NH4+ influx in roots of 3-month-old hydroponically grown Citrus (Citrus sinensis L. Osbeck x Poncirus trifoliata Blanco) seedlings. The 15NH4+ influx is saturable below an external ammonium concentration of 1 mM, indicating the action of a high-affinity transport system (HATS). The HATS is under feedback repression by the N status of the plant, being downregulated in plants adequately supplied with N during growth, and up-regulated by N-starvation. When assayed between 1 and 50 mM [15NH4+]0, the 15NH4+ influx showed a linear response typical of a low-affinity transport system (LATS). The activity of the LATS increased in plants supplied with NH4+ as compared with plants grown on an N-free medium. Transfer of the plants to N-free solution resulted in a marked decrease in the LATS-mediated 15NH4+ influx. Accordingly, resupply of NH4+ after N-starvation triggered a dramatic stimulation of the activity of the LATS. These data provide evidence that in Citrus plants, the LATS or at least one of its components is inducible by NH4+. Even when up-regulated, both the HATS and the LATS displayed a limited capacity, as compared with that usually found in herbaceous species. The use of various metabolic uncouplers or inhibitors indicated that 15NH4+ influx mediated by the HATS is strongly dependent on energy metabolism and H transmembrane electrochemical gradient. By contrast, the LATS is not affected by protonophores or inhibitors of the H+-ATPase, suggesting that its activity is mostly driven by the NH4+/NH3 transmembrane gradient. In agreement with these hypotheses, the HATS-mediated 15NH4+ influx was strongly inhibited when the solution pH was raised from 4 to 7, whereas influx mediated by the LATS was slightly stimulated.
Affiliations:
- Espagne, France
- Communauté valencienne, Languedoc-Roussillon, Occitanie (région administrative)
- Montpellier
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Pascal:02-0149830Le document en format XML
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Cerezo</name><affiliation wicri:level="2"><inist:fA14 i1="01"><s1>Unidad de Fisiología Vegetal, Departamento de Ciencias Expérimentales, Escuela Superior de Tecnología y Ciencias Experimentales, Universitat Jaume I</s1><s2>12071 Castellón</s2><s3>ESP</s3><sZ>1 aut.</sZ><sZ>5 aut.</sZ></inist:fA14><country>Espagne</country><placeName><region nuts="2" type="communauté">Communauté valencienne</region></placeName></affiliation></author><author><name sortKey="Tillard, P" sort="Tillard, P" uniqKey="Tillard P" first="P." last="Tillard">P. Tillard</name><affiliation wicri:level="3"><inist:fA14 i1="02"><s1>Biochimie et Physiologie Moléculaire des plantes, UMR 5004, Agro-M, CNRS, INRA, UM2, Place Viala</s1><s2>34060 Montpellier</s2><s3>FRA</s3><sZ>2 aut.</sZ><sZ>3 aut.</sZ></inist:fA14><country>France</country><placeName><region type="region" nuts="2">Occitanie (région administrative)</region><region type="old region" nuts="2">Languedoc-Roussillon</region><settlement type="city">Montpellier</settlement></placeName></affiliation></author><author><name sortKey="Gojon, A" sort="Gojon, A" uniqKey="Gojon A" first="A." last="Gojon">A. Gojon</name><affiliation wicri:level="3"><inist:fA14 i1="02"><s1>Biochimie et Physiologie Moléculaire des plantes, UMR 5004, Agro-M, CNRS, INRA, UM2, Place Viala</s1><s2>34060 Montpellier</s2><s3>FRA</s3><sZ>2 aut.</sZ><sZ>3 aut.</sZ></inist:fA14><country>France</country><placeName><region type="region" nuts="2">Occitanie (région administrative)</region><region type="old region" nuts="2">Languedoc-Roussillon</region><settlement type="city">Montpellier</settlement></placeName></affiliation></author><author><name sortKey="Primo Millo, E" sort="Primo Millo, E" uniqKey="Primo Millo E" first="E." last="Primo-Millo">E. Primo-Millo</name><affiliation wicri:level="2"><inist:fA14 i1="03"><s1>Departamento de Citricultura, Instituto Valenciano de Investigaciones Agrarias, Apartado Oficial</s1><s2>46113 Moncada Valencia</s2><s3>ESP</s3><sZ>4 aut.</sZ></inist:fA14><country>Espagne</country><placeName><region nuts="2" type="communauté">Communauté valencienne</region></placeName></affiliation></author><author><name sortKey="Garcia Agustin, P" sort="Garcia Agustin, P" uniqKey="Garcia Agustin P" first="P." last="Garcia-Agustin">P. Garcia-Agustin</name><affiliation wicri:level="2"><inist:fA14 i1="01"><s1>Unidad de Fisiología Vegetal, Departamento de Ciencias Expérimentales, Escuela Superior de Tecnología y Ciencias Experimentales, Universitat Jaume I</s1><s2>12071 Castellón</s2><s3>ESP</s3><sZ>1 aut.</sZ><sZ>5 aut.</sZ></inist:fA14><country>Espagne</country><placeName><region nuts="2" type="communauté">Communauté valencienne</region></placeName></affiliation></author></analytic><series><title level="j" type="main">Planta</title><title level="j" type="abbreviated">Planta</title><idno type="ISSN">0032-0935</idno><imprint><date when="2001">2001</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Planta</title><title level="j" type="abbreviated">Planta</title><idno type="ISSN">0032-0935</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Absorption</term><term>Ammonium</term><term>Biological Transport (physiology)</term><term>Biological transport</term><term>Carrier protein</term><term>Characterization</term><term>Citrus (physiology)</term><term>Concentration gradient</term><term>Deficiency</term><term>Down-Regulation</term><term>Energy metabolism</term><term>High affinity site</term><term>Hydrogen-Ion Concentration</term><term>Hydroponics</term><term>Ionophores</term><term>Isotope labelling</term><term>Kinetics</term><term>Low affinity site</term><term>Nitrates</term><term>Nitrogen Isotopes</term><term>Nutrition</term><term>Plant Roots (physiology)</term><term>Proton</term><term>Proton gradient</term><term>Proton-Translocating ATPases (antagonists & inhibitors)</term><term>Quaternary Ammonium Compounds (administration & dosage)</term><term>Quaternary Ammonium Compounds (antagonists & inhibitors)</term><term>Quaternary Ammonium Compounds (metabolism)</term><term>Regulation(control)</term><term>Root</term><term>Up-Regulation</term></keywords><keywords scheme="MESH" type="chemical" qualifier="administration & dosage" xml:lang="en"><term>Quaternary Ammonium Compounds</term></keywords><keywords scheme="MESH" type="chemical" qualifier="antagonists & inhibitors" xml:lang="en"><term>Proton-Translocating ATPases</term><term>Quaternary Ammonium Compounds</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Quaternary Ammonium Compounds</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Ionophores</term><term>Nitrogen Isotopes</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Biological Transport</term><term>Citrus</term><term>Plant Roots</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Down-Regulation</term><term>Hydrogen-Ion Concentration</term><term>Hydroponics</term><term>Kinetics</term><term>Up-Regulation</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Caractérisation</term><term>Régulation</term><term>Transport biologique</term><term>Marquage isotopique</term><term>Protéine transport</term><term>Site haute affinité</term><term>Absorption</term><term>Site faible affinité</term><term>Nutrition</term><term>Ammonium</term><term>Nitrate</term><term>Proton</term><term>Déficit</term><term>Métabolisme énergétique</term><term>Gradient proton</term><term>Gradient concentration</term><term>Racine</term><term>Citrus sinensis Poncirus trifoliata</term><term>Azote 15</term></keywords><keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Nutrition</term><term>Déficit</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">We have investigated both the kinetics and regulation of <sup>15</sup>NH<sup>4+</sup> influx in roots of 3-month-old hydroponically grown Citrus (Citrus sinensis L. Osbeck x Poncirus trifoliata Blanco) seedlings. The <sup>15</sup>NH<sub>4</sub><sup>+</sup> influx is saturable below an external ammonium concentration of 1 mM, indicating the action of a high-affinity transport system (HATS). The HATS is under feedback repression by the N status of the plant, being downregulated in plants adequately supplied with N during growth, and up-regulated by N-starvation. When assayed between 1 and 50 mM [<sup>15</sup>NH<sub>4</sub><sup>+</sup>]<sub>0</sub>, the 15NH4+ influx showed a linear response typical of a low-affinity transport system (LATS). The activity of the LATS increased in plants supplied with NH<sub>4</sub><sup>+</sup> as compared with plants grown on an N-free medium. Transfer of the plants to N-free solution resulted in a marked decrease in the LATS-mediated 15NH4+ influx. Accordingly, resupply of NH<sub>4</sub><sup>+</sup> after N-starvation triggered a dramatic stimulation of the activity of the LATS. These data provide evidence that in Citrus plants, the LATS or at least one of its components is inducible by NH<sub>4</sub><sup>+</sup>. Even when up-regulated, both the HATS and the LATS displayed a limited capacity, as compared with that usually found in herbaceous species. The use of various metabolic uncouplers or inhibitors indicated that 15NH4+ influx mediated by the HATS is strongly dependent on energy metabolism and H transmembrane electrochemical gradient. By contrast, the LATS is not affected by protonophores or inhibitors of the H<sup>+</sup>-ATPase, suggesting that its activity is mostly driven by the NH<sub>4</sub><sup>+</sup>/NH<sub>3</sub> transmembrane gradient. In agreement with these hypotheses, the HATS-mediated 15NH4+ influx was strongly inhibited when the solution pH was raised from 4 to 7, whereas influx mediated by the LATS was slightly stimulated.</div></front></TEI><affiliations><list><country><li>Espagne</li><li>France</li></country><region><li>Communauté valencienne</li><li>Languedoc-Roussillon</li><li>Occitanie (région administrative)</li></region><settlement><li>Montpellier</li></settlement></list><tree><country name="Espagne"><region name="Communauté valencienne"><name sortKey="Cerezo, M" sort="Cerezo, M" uniqKey="Cerezo M" first="M." last="Cerezo">M. Cerezo</name></region><name sortKey="Garcia Agustin, P" sort="Garcia Agustin, P" uniqKey="Garcia Agustin P" first="P." last="Garcia-Agustin">P. Garcia-Agustin</name><name sortKey="Primo Millo, E" sort="Primo Millo, E" uniqKey="Primo Millo E" first="E." last="Primo-Millo">E. Primo-Millo</name></country><country name="France"><region name="Occitanie (région administrative)"><name sortKey="Tillard, P" sort="Tillard, P" uniqKey="Tillard P" first="P." last="Tillard">P. Tillard</name></region><name sortKey="Gojon, A" sort="Gojon, A" uniqKey="Gojon A" first="A." last="Gojon">A. Gojon</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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